Comparison of central laboratory HbA1c measurements obtained from a capillary collection versus a standard venous whole blood collection in the GRADE and EDIC studies

Publication Description
We compared HbA1c values obtained from capillary blood collection kits versus venous whole blood collections in study participants with type 1 or type 2 diabetes. A total of 122 subjects, 64 with type 2 diabetes participating in the Glycemia Reduction Approaches in Diabetes: A Comparative Effectiveness (GRADE) Study and 58 with type 1 diabetes from the Epidemiology of Diabetes Interventions and Complications (EDIC) Study, participated in the validation study. Capillary tubes were filled by fingerstick by the participants on the same day as the collection of venous whole blood samples in EDTA-containing test tubes and were mailed to the central laboratory. HbA1c in all samples was measured with the same high-performance liquid chromatography. GRADE participants also completed a questionnaire on the ease of performing capillary collections. Participants from 22 clinical centers (GRADE n = 5, EDIC n = 17) were between 35 and 86 years of age, with 52% male and diverse race/ethnicities. Venous HbA1c results ranged between 5.4-11.9% (35.5-106.6 mmol/mol) with corresponding capillary results ranging between 4.2-11.9% (22.4-106.6 mmol/mol). The venous and capillary results were highly correlated (R2 = 0.993) and 96.7% differed by ≤0.2% (2.2 mmol/mol). Of participants surveyed, 69% indicated that the instructions and collection were easy to follow and 97% felt the collection method would be easy to do at home. The capillary blood HbA1c results compared well with the conventional venous whole blood results. The capillary kits can be employed in other studies to reduce interruption of critical data collection and potentially to augment clinical care when in-person visits are not possible.

Primary Author
Nathan,David M.
Krause-Steinrauf,Heidi
Braffett,Barbara H.
Arends,Valerie L.
Younes,Naji
McGee,Paula
Lund,Claire
Johnson,Mary
Lorenzi,Gayle
Gao,Xiaoyu
Steffes,Michael W.
Lachin,John M.

Volume
16

Issue
11

Other Pages
e0257154

Publisher
Public Library of Science

URL
https://www.ncbi.nlm.nih.gov/pubmed/34780485

PMID
34780485



Reference Type
Journal Article

Periodical Full
PloS one

Publication Year
2021

Place of Publication
United States

ISSN/ISBN
1932-6203

Document Object Index
10.1371/journal.pone.0257154